Glycogen Turnover in the Isolated Working Rat Heart
نویسندگان
چکیده
منابع مشابه
Preferential oxidation of glycogen in isolated working rat heart.
We tested the hypothesis that glycogen is preferentially oxidized in isolated working rat heart. This was accomplished by measuring the proportion of glycolytic flux (oxidation plus lactate production) specifically from glycogen which is metabolized to lactate, and comparing it to the same proportion determined concurrently from exogenous glucose during stimulation with epinephrine. After prela...
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INTRODUCTION Fasting increases myocardial glycogen content and has been shown to limit injury and improve recovery following no-flow ischaemia in the isolated heart. However, the protective role of glycogen loading per se in fed animals has been questioned by data in preconditioned animals showing that reduced glycogenolysis may be protective prior to no-flow ischemia. Therefore, we hypothesize...
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To what extent does glutamine turnover keep pace with oxidative metabolism in the rat heart? To address this question, the following groups of substrates were presented to the isolated, working rat heart: 1) glucose (5 mM), insulin (40 microU/ml), and [2-13C]acetate (5 mM; high workload, n = 5); 2) pyruvate (2.5 mM) and [2-13C]acetate (5 mM; normal workload, n = 5); or 3) propionate (1 mM) and ...
متن کاملAssessment of glycogen turnover in aerobic, ischemic, and reperfused working rat hearts.
Glycogen and its turnover are important components of myocardial glucose metabolism that significantly impact on postischemic recovery. We developed a method to measure glycogen turnover (rates of glycogen synthesis and degradation) in isolated working rat hearts using [3H]- and [14C]glucose. In aerobic hearts perfused with 11 mM glucose, 1.2 mM palmitate, and 100 μU/ml insulin, rates of glycog...
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Incorporation and release of the radioactivity in the liver glycogen of 18.5- and 19.5-day-old rat foetuses were studied after intravenous injection of E11-14C]glycerol. Incorporation occurred during 1 h after injection of the radioactive tracer to the foetus; then, the incorporated radioactivity decreased. Glycogen content in the liver, and glycogen phosphorylase and glycogen synthase were not...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1995
ISSN: 0021-9258
DOI: 10.1074/jbc.270.16.9234